Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
Mitochondrial
DNA (mtDNA) has been proposed to be involved in carcinogenesis and
ageing. The mtDNA 4977 bp deletion is one of the most frequently
observed mtDNA mutations in human tissues and may play a role in breast
cancer (BC). The aim of this study was to investigate the frequency of
mtDNA 4977 bp deletion in BC tissue and its association with clinical
factors. We determined the presence of the 4977 bp common deletion in
cancer and normal paired tissue samples from 106 Vietnamese patients
with BC by sequencing PCR products. The mtDNA 4977 bp deletion was
significantly more frequent in normal tissue in comparison with paired
cancer tissue. Moreover, the incidence of the 4977 bp deletion in BC
tissue was significantly higher in patients with estrogen receptor (ER)
positive as compared with ER negative BC tissue. Preliminary results
showed, in cancerous tissue, a significantly higher incidence of novel
deletions in the group of patients with lymph node metastasis in
comparison with the patients with no lymph node metastasis. We have
found 4977 bp deletion in mtDNA to be a common event in BC and with
special reference to ER positive BC. In addition, the novel deletions
were shown to be related to lymph node metastasis. Our finding may
provide complementary information in prediction of clinical outcome
including metastasis, recurrence and survival of patients with BC
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